Cheerilee is an Earth mare and the teacher of the Cutie Mark Crusaders, Dinky, Featherweight, Diamond Tiara, Silver Spoon, Twist, and Pipsqueak. She is about the same age as Big Mac.
Cheerilee has the average height, weight and face shape of an Earth mare. She has magenta fur, green eyes, and a slightly curly, pink-striped mane and tail. Her cutie mark is three smiling flowers.
Cheerilee is a very kind and devoted teacher, having earned her cutie mark upon being hired as a teacher (as a teen), the three flowers represent her wish to help the students bloom, and the smiles represent how she wants them to be happy. She is single but doesn't mind, although she did briefly fall in love with Big Mac, but that was due to Love Poisoning.
- As a teen, her mane and tail were frizzy, and apparently, so were everypony else's in her class.